利用金屬奈米電漿子進行核酸擴增和即時定量之SARS-CoV-2檢測平台
SARS-CoV-2 sensing platform using metallic nanoplasmonics for nuclear acid amplification and real-time quantification
計畫主持人：臺大-應力所 沈弘俊、中研院-應科中心 魏培坤
新型冠狀病毒為具有強大的傳染性的病毒，近年來新型冠狀病毒已經蔓延至全球各個角落，即使疫苗的覆蓋率高，但感染後症狀不明顯，且其症狀也與一般感冒極為類似，容易遭到誤判而忽略，導致病人健康出現危險，再加上近期流感病毒的演進，讓醫療團隊在辨識新型冠狀病毒與流感病毒變得異常困難，在此背景下，精準檢測的SPR快篩裝置開發變得尤為重要。
本研究團隊透過吸入式的腔體設計，將流道與檢測晶片相接，降低檢測的誤差、時間與樣品溶液的用量，此外，也透過設計不同長度的DNA模板、引子與探針，調配成PCR溶液並最佳化實驗環境參數，讓檢測樣品有更好的接合效果。該裝置不僅能減少寶貴醫療資源和人力的浪費，並保護醫護人員的安全，還能在短時間內顯示正確的檢測結果，為醫療人員提供快速決策的依據。
The novel coronavirus is a highly infectious virus that has spread globally in recent years. Despite high vaccination coverage, the symptoms post-infection are often subtle and similar to those of the common cold, leading to potential misdiagnosis and health risks. The evolution of influenza viruses has further complicated the differentiation between coronavirus and influenza, making the development of accurate detection devices like the SPR rapid screening device crucial.
The research team has developed an inhalation-based chamber design, integrating the flow channel with the detection chip to reduce detection errors, time, and the amount of sample solution needed. In addition, by designing DNA templates, primers and probes of varying lengths, they prepared PCR solutions and optimized experimental environment parameters to achieve better sample bonding efficiency. This research holds significant importance in the biomedical and healthcare fields, especially in enhancing detection accuracy and accelerating the diagnostic process.